Sophie Archambeault
- Research Assistant & Volunteer
- Center for Comparative Genomics
Entomology & CABI
SOPHIE LOUISE ARCHAMBEAULT
sarchambeault@calacademy.org
WORK EXPERIENCE
University of California, San Francisco, San Francisco, CA. July 2005 – June 2008, December 2008 - Present
Staff Research Associate II & III
• Planned and conducted experiments; interpreted results and organized data for projects including routine genotyping of human leukemic samples, granulocyte/monocyte colony stimulating factor (GM-CSF) hypersensitivity screening, and minimal residual disease (MRD) detection using TaqMAMA.
• Developed and implemented new assays for the laboratory including TaqMAMA for MRD detection, sequencing on the ABI 3100 for routine in-house genotyping, Taqman SNP genotyping for allele detection and the long-term culture-initiating cell assay.
• Acted as laboratory contact person and liaison between Environmental Health and Safety and 20+ laboratory members, responded to safety audit reports and kept up to date radioactivity records.
• Maintained, provided training and performed troubleshooting on diverse instruments and software, especially the ABI 7900 Real Time PCR Instrument, ABI 3100 Sequencer, and Sequencher 4.6.
California Academy of Sciences, San Francisco, CA. February 2009 - Present
Molecular Laboratory Volunteer and part-time Laboratory Assistant
• Extracted DNA and performed cycle sequencing to investigate the phylogenetic relationships of Nebria beetles.
• Assisted in laboratory maintenance, including bi-monthly billing for laboratory users.
University of California, San Francisco, San Francisco, CA. August 2003 - April 2005
Staff Research Associate I & II
• Processed Escherichia coli samples including storage preparation, isolation of genomic DNA, PCR amplification of target genes, and automated sequencing of PCR products.
• Synthesized, purified and analyzed custom oligonucleotides for core facility, trained new employees and interacted with customers for delivery and troubleshooting.
• Performed routine maintenance and troubleshooting of robots, DNA synthesizers and HPLC machines.
• Reviewed and complied with routine laboratory audits as Laboratory Safety Officer.
• Maintained synthesis records, machine maintenance records, customer order forms and oligonucleotide analytical reports; ordered reagents and set up standing order to keep laboratory stocked.
• Performed duties related to DNA sequencing service including preparing DNA templates, pipetting DNA sequencing reactions, operating the ABI 3700 DNA sequencer, data analysis and customer service.
• Assisted in Taqman Quantitative PCR service including pipetting 384-well reaction plates, operating the ABI 7900 Taqman machine and delivering data to customers.
Mystic Aquarium, Mystic, CT. Fall 2000, 2001, 2002, Spring 2003
Animal Husbandry/Stranding/Laboratory Volunteer
• Prepared food, fed whales, participated in animal training and cleaned backstage area.
• Assisted with routine physicals and restraint for stranded seals.
• Measured pH, salinity, ORP, iodide, magnesium, aluminum and nitrate of water samples from tanks; recorded data in Excel spreadsheets.
Center for Reproduction of Endangered Species, Escondido, CA . Summer 2002
Bonner Summer College Fellow
• Reviewed literature on the phylogenetic relationships of New World monkeys.
• Extracted, PCR amplified and sequenced DNA from frozen tissue samples of New World monkeys.
• Aligned and analyzed sequences using PAUP 4.0b9.
PUBLICATIONS
Mignon L. Loh*, Debbie S. Sakai, Christian Flotho, Michelle Kang, Manfred Fliegauf, Sophie Archambeault, Charles G. Mullighan, Leslie Chen, Eva Bergstraesser, Carlos E. Bueso-Ramos, Peter D. Emanuel, Henrik Hasle, Jean-Pierre Issa, Marry M. van den Heuvel-Eibrink, Franco Locatelli, Jan Stary, Monica Trebo, Marcin Wlodarski, Marco Zecca, Kevin M. Shannon, and Charlotte M. Niemeyer. Mutations in CBL occur frequently in juvenile myelomonocytic leukemia. Blood. 2009.
Kotecha, N., N.J. Flores, J.M. Irish, E.F. Simonds, D.S. Sakai, S. Archambeault, E. Diaz-Flores, M. Coram, K.M. Shannon, G.P. Nolan, M.L. Loh. Single-Cell Profiling Identifies Aberrant STAT5 Activation in Myeloid Malignancies with Specific Clinical and Biologic Correlates. Cancer Cell. 2008.
Berman, J.N., W.L. Greer, S. Archambeault, M.L. Loh, C. Riddell, B. Morash, N. Dumas, C.V. Fernandez, M.D. Ludman. JAK2 V617F Positive Polycythemia Vera in a Child with Neurofibromatosis Type I. Pediatric Blood Cancer. 2008.
Archambeault, S., N.J. Flores, A. Yoshimi, C. Kratz, M. Reising, A. Fischer, P. Nöllke, F. Locatelli, P. Sedlacek, C. Flotho, M. Zecca P. Emanuel, R. Castleberry, C. Niemeyer, P. Bader, M. Loh. Development of an Allele-specific Minimal Residual Disease Assay for Patients with Juvenile Myelomonocytic Leukemia. Blood. 2008.
RESEARCH PRESENTATIONS
Archambeault, S., A. Yoshimi, C. Kratz, M. Reising, A. Fischer, P. Nöllke, F. Locatelli, P. Sedlacek, C. Flotho, M. Zecca P. Emanuel, R. Castleberry, C. Niemeyer, P. Bader, M. Loh. Development of an Allele-specific Minimal Residual Disease Assay for Patients with Juvenile Myelomonocytic Leukemia—Moving Beyond Clinical Assessment. Annual Meeting of the American Society of Hematology, December 8-12, 2006. Orlando, FL.
Ivanetich, K.M., S. Archambeault, P.-h. Hsu and B. Finkmoore. Development of DNA Sequencing Assays to Identify and Differentiate Host Sources of Fecal Pollution. USDA-CSREES Water Quality Conference, February 7-9, 2005. San Diego, CA.
Barnes, P.T., S.L. Archambeault and E.E. Pisano. The Effect of Long-term Laboratory Culture on the Evolution of Flight in Drosophila melanogaster. Annual Meeting of the Society for the Study of Evolution, June 26-30, 2004. Colorado State University, Fort Collins, CO.
EDUCATION
CONNECTICUT COLLEGE, New London, CT. Bachelor of Arts, May 2003
Major: Zoology G.P.A. 3.9
Honors: Phi Beta Kappa, May 2003; Deans Honors, Deans High Honors all semesters; Sybil A. Hausman Award for outstanding work in the Zoology department, 2002; Jewel H. and James T. Bonner & The Werner Heuschele Summer College Student Fellowship from the Center for Reproduction of Endangered Species, Summer 2002; Lawrence Scholar, 1999.
Relevant Courses: General Biology, Molecular Biology, Cell Biology, Vertebrate Biology, General Chemistry, Organic Chemistry, General Zoology, Wildlife Ecology, General Physics, Calculus III, Bioethics, Philosophy of Biology.
THE SCHOOL FOR FIELD STUDIES, Center for Wildlife Management Studies, Kenya January 2002 - May 2002.
ACADEMIC RESEARCH AND INDEPENDENT STUDY
Genetics Department, Connecticut College, New London, CT. Fall 2002 - Spring 2003
Honors thesis student
• Reviewed existing literature on life history trade-offs.
• Carried out research on flight abilities of Drosophila melanogaster.
FAMILIAR TECHNIQUES
Automated DNA sequencing, DNA extraction from blood and tissue, DNA Amplification by Polymerase Chain Reaction (PCR), Real Time Quantitative PCR, Colony Forming Unit Assay, Long-Term Culture-Initiating Cell Assay, Western Blotting, GeneChip Human Mapping 100K Set, Single Strand Conformational Polymorphism PCR, Reverse Transcription PCR, Gel Electrophoresis, Yeast transformation, Scanning Electron Microscopy, Yeast two-hybrid System, Taqman SNP Genotyping.
COMPUTER SKILLS
Microsoft Word • PowerPoint • Excel • FileMaker Pro • SPSS for Windows • Sequencher 4.6 • PAUP 4.0b9
Familiar with PCs • Macs • MS DOS
INTERESTS & HOBBIES
Hiking • SCUBA diving • Orchestra • Biking • Sewing • Surfing • Traveling
Mignon L. Loh, Debbie S. Sakai, Christian Flotho, Michelle Kang, Manfred Fliegauf, Sophie Archambeault, Charles G. Mullighan, Leslie Chen, Eva Bergstraesser, Carlos E. Bueso-Ramos, Peter D. Emanuel, Henrik Hasle, Jean-Pierre Issa, Marry M. van den Heuvel-Eibrink, Franco Locatelli, Jan Stary, Monica Trebo, Marcin Wlodarski, Marco Zecca, Kevin M. Shannon, and Charlotte M. Niemeyer. Mutations in CBL occur frequently in juvenile myelomonocytic leukemia. Blood. 2009.
Kotecha, N., N.J. Flores, J.M. Irish, E.F. Simonds, D.S. Sakai, S. Archambeault, E. Diaz-Flores, M. Coram, K.M. Shannon, G.P. Nolan, M.L. Loh. Single-Cell Profiling Identifies Aberrant STAT5 Activation in Myeloid Malignancies with Specific Clinical and Biologic Correlates. Cancer Cell. 2008.
Berman, J.N., W.L. Greer, S. Archambeault, M.L. Loh, C. Riddell, B. Morash, N. Dumas, C.V. Fernandez, M.D. Ludman. JAK2 V617F Positive Polycythemia Vera in a Child with Neurofibromatosis Type I. Pediatric Blood Cancer. 2008.
Archambeault, S., N.J. Flores, A. Yoshimi, C. Kratz, M. Reising, A. Fischer, P. Nöllke, F. Locatelli, P. Sedlacek, C. Flotho, M. Zecca P. Emanuel, R. Castleberry, C. Niemeyer, P. Bader, M. Loh. Development of an Allele-specific Minimal Residual Disease Assay for Patients with Juvenile Myelomonocytic Leukemia. Blood. 2008.