| Family | genus | species | author | Type Number | Category |
| Drosophilidae | Drosophila | acuminanus | Hunter | 15852 | holotype |
| Drosophilidae | Drosophila | anuda | Curran | 4055 | holotype |
| Drosophilidae | Drosophila | arane | Hunter | 15853 | holotype |
| Drosophilidae | Drosophila | arboloco | Hunter | 13472 | holotype? |
| Drosophilidae | Drosophila | bifurcada | Hunter | 15854 | holotype |
| Drosophilidae | Drosophila | bomarea | Hunter | 13473 | holotype? |
| Drosophilidae | Drosophila | carablanca | Hunter | 13474 | holotype? |
| Drosophilidae | Drosophila | chisaca | Hunter | 15855 | holotype |
| Drosophilidae | Drosophila | choachi | Hunter | 15856 | holotype |
| Drosophilidae | Drosophila | colmenares | Hunter | 15857 | holotype |
| Drosophilidae | Drosophila | crockeri | Curran | 4057 | holotype |
| Drosophilidae | Drosophila | desbaratabaile | Hunter | 13475 | holotype? |
| Drosophilidae | Drosophila | equinoxialis caribbensis | Ayala | 11787 | holotype |
| Drosophilidae | Drosophila | franii | Hunter | 15858 | holotype |
| Drosophilidae | Drosophila | freilejoni | Hunter | 13476 | holotype? |
| Drosophilidae | Drosophila | margarita | Hunter | 13477 | holotype? |
| Drosophilidae | Drosophila | mitis | Curran | 4053 | holotype |
| Drosophilidae | Drosophila | pseudoobscura bogotana | Ayala and Dobzhansky | 12072 | holotype |
| Drosophilidae | Drosophila | willistoni quechua | Ayala | 11786 | holotype |
| Drosophilidae | Drosophila | willowsi | Curran | 4052 | holotype |
| Drosophilidae | Hyalistata | mexicoa | Grimaldi | 17192 | holotype |
| Drosophilidae | Leucophenga | xanthobasis | Curran | 4951 | holotype |